Archive for Fertility News
Objective: To evaluate the effects on human spermatozoa of freeze-drying, also known as lyophilization, and of cryopreservation in liquid nitrogen.Design: Prospective experimental study.Setting: Reproductive medicine unit and a private IVF center.Patient(s): Thirty healthy male donors.Intervention(s): Sperm samples from 30 donors divided as two aliquots, one to be lyophilized and the other to be cryopreserved in [...]
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Posted on April 28, 2012 | Filed under Fertility News | Permalink
Objective: To report a case of successful testicular sperm extraction performed during an emergency scrotal exploration for trauma in a patient with a history of previous contralateral cryptorchidism.Design: Case report.Setting: Clinica Urologica I, Università degli Studi di Milano, Fondazione Istituto di Ricerca e Cura a Carattere Scientifico Ca’ Granda, Ospedale Maggiore Policlinico.Patient(s): A 28-year-old man [...]
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Posted on April 28, 2012 | Filed under Fertility News | Permalink
Objective: To determine whether metabolomic analysis of follicular fluid could prove a useful noninvasive technique for the selection of viable oocytes and embryos.Design: Metabolomic analysis based on proton nuclear magnetic resonance (1H NMR) performed on follicular fluid collected from in vitro fertilization (IVF) patients.Setting: A university research center and a private fertility clinic.Patient(s): Fifty-eight women undergoing [...]
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Posted on April 28, 2012 | Filed under Fertility News | Permalink
Objective: To evaluate the effect of laser polar-body biopsy (PBB) for preimplantation genetic diagnosis on embryo quality.Study design: Retrospective case-control analysis. The quality of 145 embryos after PBB was compared to 276 embryos of the same group of women without biopsy.Setting: University-based tertiary-care medical center.Patient(s): Women with inherited genetics disease.Intervention(s): Laser PBB of IVF embryos [...]
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Posted on April 28, 2012 | Filed under Fertility News | Permalink
Objective: To determine the optimal size of the leading follicle before human chorionic gonadotropin (hCG) administration in cycles with clomiphene citrate (CC) and letrozole, and to examine any differences in the optimal leading follicle size between cycles with CC and letrozole.Design: A retrospective study.Setting: University hospital-based reproductive center.Patient(s): 1,075 women undergoing intrauterine insemination cycles with [...]
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Posted on April 28, 2012 | Filed under Fertility News | Permalink
Objective: To describe the management of hydrosalpinges among Society for Reproduction Endocrinology and Infertility (SREI)/Society of Reproductive Surgeons (SRS) members.Design: Cross-sectional survey of SREI/SRS members.Setting: Academic and private practice–based reproductive medicine physicians.Participant(s): A total of 442 SREI and/or SRS members.Intervention(s): Internet-based survey.Main Outcome Measure(s): To understand how respondents evaluate, define, and manage hydrosalpinges.Result(s): Of 1,070 [...]
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Posted on April 28, 2012 | Filed under Fertility News | Permalink
Objective: To update results on outcomes with frozen/thawed oocytes using a differential sucrose concentration during dehydration (0.2 M) and rehydration (0.3 M), combined with a one-step propanediol exposure.Design: Retrospective cohort study.Setting: Private IVF centers.Patient(s): Infertile couples undergoing IVF treatment.Intervention(s): Oocyte thawing cycles between May 2004 and December 2010.Main Outcome Measure(s): Survival, fertilization, and cleavage rates were [...]
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Posted on April 28, 2012 | Filed under Fertility News | Permalink
Objective: To evaluate the effect of recombinant LH in assisted reproduction technology (ART) cycles in patients of advanced reproductive age.Design: A systematic review and meta-analysis.Setting: Published randomized controlled clinical trials comparing recombinant LH plus recombinant FSH versus recombinant FSH only in patients of advanced reproductive age.Patient(s): Patients 35 years and older undergoing assisted reproduction.Intervention(s): Recombinant [...]
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Posted on April 28, 2012 | Filed under Fertility News | Permalink
Objective: To investigate the role of activin-?A subunit, activin type II receptors, inducible nitric oxide synthase (iNOS), and MUC1 in the pathogenesis of ectopic pregnancy (EP) and their involvement in the determination of the implantation site.Design: Observational study.Setting: Academic unit of reproductive and developmental medicine.Patient(s): Four women at the luteal phase, three pseudopregnant women at [...]
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Posted on April 28, 2012 | Filed under Fertility News | Permalink
Objective: To consider a possible cumulative effect of two genetic polymorphisms (FOXP3 C-2383T/rs3761549 and FCRL3 C-169T/rs7528684) that were previously shown to be associated with endometriosis.Design: Genetic association study.Setting: Human reproduction outpatient clinic of Faculdade de Medicina do ABC.Patient(s): One hundred eighty-eight infertile women with endometriosis and 169 controls.Intervention(s): Detection of polymorphisms FOXP3 (C-2383T/rs3761549) and FCRL3 [...]
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Posted on April 28, 2012 | Filed under Fertility News | Permalink
Objective: To investigate the expression and regulation of colony-stimulating factor 1 (CSF-1) and its receptor, C-FMS, in endometriosis.Design: In vivo and vitro study.Setting: University-based academic medical center.Patient(s): Reproductive-age women undergoing surgery for benign conditions.Intervention(s): Peritoneal and endometrial tissue samples were obtained.Main Outcome Measure(s): CSF-1 and C-FMS expression.Result(s): Significantly higher CSF-1 levels were found in peritoneal fluid [...]
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Posted on April 28, 2012 | Filed under Fertility News | Permalink
Objective: To investigate the association between leisure-time physical activity (PA) and fecundability.Design: Prospective cohort study.Setting: Internet-based observational study of Danish women who were planning a pregnancy (2007–2009).Patient(s): A total of 3,628 women aged 18–40 years at baseline.Intervention(s): None.Main Outcome Measure(s): Time to pregnancy (TTP). Fecundability ratios (FRs) and 95% confidence intervals (CIs) were derived from [...]
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Posted on April 28, 2012 | Filed under Fertility News | Permalink
Objective: To investigate the occurrence of a gene–environment interaction between glutathione transferase (GST) gene polymorphisms (GSTM1, GSTT1, GSTP1, and GSTA1) and serum polychlorinated biphenyls (PCBs) levels. This is suggested as possible risk factors for endometriosis, a multifactorial gynecological disease.Design: Case-control study conducted from 2002 to 2005.Setting: Policlinico Umberto I, “Sapienza” University of Rome and Italian National [...]
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Posted on April 28, 2012 | Filed under Fertility News | Permalink
Objective: To investigate whether solid-surface vitrification (SSV) is an effective cryopreservation strategy regarding the integrity and function of prepubertal mouse testicular tissue.Design: Prospective experimental study.Setting: Academic research unit.Animal(s): Mice.Intervention(s): Testicular tissue from 5- to 10-day-old GFP+ mice was cryopreserved with the use of a conventional uncontrolled slow freezing (USF) technique and SSV before intratesticular grafting [...]
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Posted on April 28, 2012 | Filed under Fertility News | Permalink
Objective: To identify molecular markers associated with adhesion and normal peritoneal tissue using microarray expression profiling.Design: Comparative study.Setting: University hospital.Patient(s): Five premenopausal women.Intervention(s): Adhesion and normal peritoneal tissue samples were obtained from premenopausal women. Ribonucleic acid was extracted using standard protocols and processed for hybridization to Affymetrix Whole Transcript Human Gene Expression Chips. Microarray data [...]
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Posted on April 28, 2012 | Filed under Fertility News | Permalink
Objective: To study the cause for poor oocyte yield, amenorrhea, and recurrent pregnancy loss in a patient undergoing IVF.Design: Case report.Setting: University-affiliated private IVF clinic.Patient(s): A 33-year-old woman with amenorrhea, recurrent ovarian cyst formation, poor oocyte yield, and repeated chemical pregnancies after IVF treatments.Intervention(s): The hCG stimulation test and luteinizing hormone/chorionic gonadotropin receptor (LHCGR) gene [...]
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Posted on April 28, 2012 | Filed under Fertility News | Permalink
Objective: To study women with a poor response to ovarian hormone stimulation, known as low responders. Genetic defects in the FSH receptor gene (FSHR) were analyzed as well as antimüllerian hormone (AMH) for ovarian reserve.Design: Retrospective cohort study.Setting: University hospital.Patient(s): Two hundred fifty-nine patients total: 74 low responders; 88 patients receiving assisted reproduction therapy (ART) [...]
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Posted on April 28, 2012 | Filed under Fertility News | Permalink
Objective: To investigate four different protocols for cryopreservation of the whole rat ovary with intact vasculature to evaluate whether differences exist in post-thawing viability of the ovary after either vitrification or slow freezing.Design: Experimental study.Setting: Obstetrics and gynecology department.Animal(s): Immature Sprague-Dawley female rats.Intervention(s): Ovaries were isolated with the vascular tree intact up to the bifurcation [...]
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Posted on April 28, 2012 | Filed under Fertility News | Permalink
Objective: To demonstrate whether leptin modulates reproduction by a direct effect within the ovary.Design: Animal model.Setting: National Key Laboratory of Infertility.Animal(s): Adult female db/db mice.Intervention(s): Adult littermate wild-type (WT) and diabetic (db) leptin receptor (LR) mutant female mice were matched for the allograft of the ovary to construct new genotypic models, respectively. WT mouse received [...]
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Posted on April 28, 2012 | Filed under Fertility News | Permalink
Objective: To determine the feasibility of bioluminescence imaging (BLI) to evaluate the efficiency of germ cell transplantation in vitro and in vivo in fertility preservation for infertile male mice.Design: Transgenic mouse model.Setting: University-based teaching hospital.Animal(s): Transgenic mice.Intervention(s): Busulfan was used to induce testicular failure in 3-week-old immature FVB/NJNarl wild-type recipient mice. At 8 weeks of age they [...]
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Posted on April 28, 2012 | Filed under Fertility News | Permalink
Objective: To investigate the role of arachidonic acid (AA) in mouse endometrial stromal cells.Design: Experimental animal study.Setting: University research laboratory.Animal(s): Sexually mature female CD1-strain mice.Intervention(s): Primary culture of endometrial stromal cells.Main Outcome Measure(s): Western blot and real-time polymerase chain reaction for gene expression and/or phophorylation analysis. Luciferase assay for Cox-2 promoter analysis.Result(s): AA-derived prostaglandins play [...]
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Posted on April 28, 2012 | Filed under Fertility News | Permalink
Objective: To investigate the location of ?-1,4-N-acetylgalactosaminyltransferase II (B4GALNT2) and the involvement of this protein and Sda antigen in embryonic implantation.Design: Cell and animal study.Setting: University.Animal(s): Adult outbred Institute for Cancer Research mice.Intervention(s): B4GALNT2 antibody injected into the uteri of mice in early pregnancy; E3.5 blastocysts and pregnant uterine tissues were collected.Main Outcome Measure(s): Protein [...]
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Posted on April 28, 2012 | Filed under Fertility News | Permalink
Objective: To investigate the presence of neurokinin B (NKB)/NK3 receptor (NK3R) and kisspeptin/KISS1 receptor (KISS1R) messenger RNA (mRNA) and proteins throughout the human female genital tract.Design: In vitro study.Setting: Academic research laboratories and academic hospitals.Patient(s): Fifteen reproductive-age women and 16 postmenopausal women provided fresh samples of uterus, ovary, or oviduct, and 12 women provided archival samples [...]
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Posted on April 28, 2012 | Filed under Fertility News | Permalink
Objective: To evaluate the efficiency of slush nitrogen vitrification of human oocytes with or without cumulus cells in terms of survival rate and maintenance of meiotic spindle.Design: Randomized, comparative study.Setting: Medical center.Patient(s): A total of 274 oocytes obtained from 46 couples undergoing infertility treatment.Intervention(s): Metaphase II oocytes were divided into groups A and B, vitrified [...]
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Posted on April 28, 2012 | Filed under Fertility News | Permalink
Objective: To show that antibodies against ACTL7a, a spermatozoon-specific protein, may be a cause of immunologic infertility.Design: Determine the presence of anti-ACTL7a antibodies in infertile blood, raise antibodies against ACTL7a in rabbits, and demonstrate that the in vitro treatment of mouse spermatozoa with infertile sera markedly reduces their fertilizing capacity. Demonstrate that the active immunization of [...]
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Posted on April 28, 2012 | Filed under Fertility News | Permalink
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